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Matrix metalloproteinase-9 (MMP-9) can promote the invasion and metastasis of breast cancer through various mechanisms.The expressions of MMP-9 in the patients with breast cancer are correlated with the prognosis.The higher expression of MMP-9 indicates poorer prognosis.It is suggested that the level change of MMP-9 pre and post treatment can be used as a reference standard for judging the prognosis and clinical effect.
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Aim: To evaluate the effects of bioactive extracts from Fructus Jujubae in attenuating the inflammation induced by Radix Kansui. Methods: Exoteric splenic lymphocyte ( SPL) of mouse and peritoneal macrophage ( PMψ) of rat were used as cell model to evaluate the anti-inflammatory effects of fractions from Fructus Jujubae. MTT method was used to assay SPL proliferation, and Griess method was used in NO release of PMψ. Firstly, Radix Kansui fraction was applied to induce inflammatory effects of the cells, and then, the anti-inflammatory effects of five extracts of Fructus Jujubae were investigated to determine the active fractions in Fructus Jujubae, which probably contribute to the attenuation of Radix Kansui. The effects of Fructus Jujubae extract in interfering the gastrointestinal acute damages caused by the extract of Radix Kansui were observed according to the pathological sections of mice. Results: The extract RK-3 ( diterpenes) exhibited the most significant pro-inflammatory effect. The bioactive extracts B (micromolecule saccharides and amino acids), D(flavonoid glycosides), and E (triterpenes) of Fructus Jujubae could attenuate the action of RK-3. The effective extract of Fructus Jujubae protected stomach and duodenum of mouse from acute damages caused by the extract of Radix Kansui. Conclusion: Fraction RK-3 (diterpenes) of Radix Kansui had pro-inflammatory effects, and some extracts of Fructus Jujubae could attenuate this effect of Radix Kansui. The results helped to clarify that Fructus Jujubae attenuates the toxic effect of Radix Kansui in certain cases, and the bioactive extracts of Fructus Jujubae discovered in the experiments offer the basis for further research to the active components of Fructus Jujubae.
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OBJECTIVE: To analyze the types and the potential causes of Danshen injection inducing ADR cases. METHODS: 302 ADR cases induced by Danshen injection were collected from ADR cases reported in Jiangsu province from 2003 to 2008 according to reported primarily susceptive drug and the description of the ADR. All cases were analyzed statistically to find out possible reason that ADR was induced by active components of Danshen injection. RESULTS: Of total 302 ADR cases, 0.66% was likely to be caused by the active components of Danshen injection; 96.36% allergic reaction were not possibly related to active components; 2.32% was caused by the drug preparation; 0.66% was caused by unclear reason. CONCLUSION: More than 90% ADR cases induced by Danshen injection manifests as allergic reaction, which isn’t possibly related to main active components. The active components could induce the hemorrhage.
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OBJECTIVE: To establish a feasible method for the clinical pharmacists of cardiovascular diseases during training stage. METHODS: Drawn from the author's experiences, the clinical features of cardiovascular diseases were analyzed and summed up. RESULTS & CONCLUSIONS: A good method is useful for the clinical cardiovascular pharmacist to carry out the medical work and become a member of clinical therapeutic team as sooner as possible.
ABSTRACT
With the development of proteomics, the application of proteomics in the research for the medicine of reproduction becomes more and more widely, especially in physiology, pathology, toxicology of re- production and drug discovery. Significant performance has been achieved for instance, PHGPx is identified in mitoehondrial capsule by proteomies approach. 53 proteins involved in the spermatogenesis of rat are identified. The toxicological effect of TCDD on placenta of rat has also been conducted. As a kind of tools of molecular biology, proteomies approach brings into full play.